Tripletof 5600 acs publications american chemical society. Development, validation and application of a qualitative. Request pdf performance characteristics of a new hybrid quadrupole timeofflight tandem mass spectrometer tripletof 5600 the tripletof 5600. In this study, a comparative uhplcqtofmsbased metabolomics approach combining equivalentquantification and the taste activity value tav was used, for the first time, to evaluate the taste qualities and characterize metabolite profiles in chinese fish sauce during fermentation. Application of uhplcqtofmsbased metabolomics in the. The inlet parameters for the source are described in figure 2. Metabolomics vladimir tolstikov, phd eli lilly and company. The recently introduced tripletof 5600 system tripletof, a hybrid triple quadrupole tof platform, is considered the first accurate mass, high resolution system of its kind and operates by means of information dependent acquisition ida with the speed and sensitivity of a tof ms and quantification capabilities similar to a triple quadrupole ms. A practical introduction to analysis of intact proteins on. The nano lcmsms analysis of proteolytically digested therapeutic antibodies level 3 and the glycan analysis level 4 will be discussed in separate technical notes and will further highlight the flexibility and advantages of the tripletof 5600 system for protein characterization.
Each cycle consisted of a tofms spectrum acquisition for 250 ms mass range 4001250 da, followed by acquisition of up to 50 msms spectra 50 ms each, mass range 652000 da of ms peaks above intensity 150 taking 2. Analysis of cardiac glycosides in suicide tree kernel. To compare the results withthose obtained in a absciex 5500 qtrap triple. Concerning your issue about the blood sample preparation, enrichment by using lcmsms tripletof 5600. Two samples from abrf sprg2011 were used for lcms and msms by ab sciex 5600 tripletof with cid fragmentation. Tofmsms parameters the analysis was performed using the product ion mode on a tripletof 5600 system. Lam7, nuno bandeira8, eric deutsch9 and thomas neubert10. The combination of high resolution, mass accuracy, sensitivity and speed of ms and msms scans using the ab sciex tripletof 5600 lcms system enabled rapid comprehensive analysis of a lipid extract in only 1 hour.
The lcms system consisted of an agilent 1100 series hplc agilent technologies, palo alto, ca,detectedusa coupled to a triple tof 5600 mass spectrometer sciex, ontario, canada equipped with a turboionspray source. Agilent 6200 series tof and 6500 series qtof lcms system. Analysis of intact and reduced therapeutic mab using. Mrm experiments performed on a triplequadruple mass spectrometry instrument were used for quantitation. Tof ms and msms data at high acquisition rates makes it a powerful tool for proteomics research. Plasma pharmacokinetic determination of canagliflozin and. Below are guidelines for ms parameters necessary to build an acquisition method for intact proteins on the tripletof 5600 system. Performance characteristics of a new hybrid quadrupole. The instrument parameters on triple tof 5600 were set at values recommended by ab sciex. Clauser2, robert j chalkley3, ruixiang sun4, eugene kapp5, matt chambers6, w. Four quantitative hyperfractionated plasma proteomics experiments were analyzed in replicates by two ab sciex tripletof 5600 and three thermo scientific orbitrap eliteltqorbitrap velosq exactive instruments. Oct 19, 2018 widespread use of the ab sciex triple tof 5600 ms ms system is attributed to its online data acquisition, which is followed by comprehensive data processing techniques including extracted ion. Ab sciex launches nextgeneration tripletof 5600 system. Tof, the ion trap it, the triple quadrupole qqq, and the quadrupole timeofflight qtof.
Agilent 6200 series tof and 6500 series qtof lcms system concepts guide 11 a superheated sheath gas to collimate the nebulizer spray which dramatically increases the number of ions that enter the mass spectrometer. Swath acquisition is the newest workflow enabled by tripletof technology, see section 5 for a list of 18 publications leveraging this data independent workflow. Identification of metabolites of liquiritin in rats by. Advanced graduate course in metabolomics 3112015 janusz kabarowski, dept. Moreover, operating the tripletof tm 5600 coupled with an ldtd tm ion source allows for an outstanding analytical speed of 4. I think the following below links may help you in your analysis. For collision induced dissociation tandem mass spectrometry cid.
Matrix molecules absorb laser light, enter an excited. A study on detecting modified peptides in a complex mixture a b r f proteome informatics research group john cottrell1, karl r. Identification of metabolites of liquiritin in rats by uhplc. The high resolution and mass accuracy on a timeofflight. Our exclusive contracts with our clients yield a wide range of used mass spectrometers from a number of respected oems, including agilent, thermo scientific, applied biosystems, waters, perkin elmer, and many more. The high resolution and accurate mass ab sciex tripletof. Matrix molecules absorb laser light, enter an excited state, and collide with sample molecules, facilitating charge transfer to create ions. This is the foundation of the groundbreaking swath acquisition that is changing the landscape of quantitative proteomics.
Following liquidliquid extraction by tertbutyl methyl ether, chromatographic separation of canagliflozin and its metabolites were performed on a waters xbridge beh c18 column 100 2. For protein identification, each sample is first analyzed with malditof ms to generate the pmf data scanning range. Quantitation of insulin analogue glargine and its two. It uniquely integrates comprehensive, qualitative exploration, rapid profiling, and highresolution quantitation workflows. Tof 5600 mass spectrometer sciex, ontario, canada equipped. Widespread use of the ab sciex triple tof 5600 msms system is attributed to its online data acquisition, which is followed by comprehensive.
Equipnet is the worlds leading provider of used mass spectrometers and numerous other types of preowned equipment. Then the five most abundant peptides precursors that are not on exclusion list are selected for further fragmentation maldi tof tof analysis to generate the full scan mass spectrum. Concerning your issue about the blood sample preparation, enrichment by using lc ms ms triple tof 5600. That is described in detail in the pdf file on page 11 and.
This individual must complete the prerequisite introduction to lcmsms operation series for sciex tripletof systems. Informationdependent acquisition ida was carried out. The sciex tripletof 5600 mass spectrometer is known for delivering excellent sensitivity and linear dynamic range. Uhplcqtofmsmsoriented characteristic components dataset. The nano lc ms ms analysis of proteolytically digested therapeutic antibodies level 3 and the glycan analysis level 4 will be discussed in separate technical notes and will further highlight the flexibility and advantages of the tripletof 5600 system for protein characterization. Run a tof ms or product ion scan type and confirm that there is a stable tic and. Pn ab sciex product description list price discount net. All product ions use 100 as declustering potential dp, an. Q exactive a true qualquan hram mass spectrometer for. For protein identification, each sample is first analyzed with maldi tof ms to generate the pmf data scanning range. Plasma proteomic experiments performed rapidly and economically using several of the latest highresolution mass spectrometers were compared. Tof ms ms parameters the analysis was performed using the product ion mode on a tripletof 5600 system.
Ab sciex tripletof 5600 nontargeted screening of an unlimited. Adme sample analysis can be performed in hr ms which avoids the need for a compound optimization step. High resolution ms extract the signal of every in order to compound of interest from a sample. One contains only a lyophilized mixture of 70 synthetic modified peptides, and the second contains the same mixture combined with a tryptic digest of the six proteins from which the synthetic peptides were derived. Immunodepletion plasma proteomics by tripletof 5600 and orbitrap eliteltqorbitrap velosq exactive mass spectrometers. Spontaneous fermentation is a critical step in the processing of highquality fish sauce. Performance characteristics of a new hybrid quadrupole time. Plasma pharmacokinetic determination of canagliflozin and its. Oct 04, 20 each cycle consisted of a tof ms spectrum acquisition for 250 ms mass range 4001250 da, followed by acquisition of up to 50 ms ms spectra 50 ms each, mass range 652000 da of ms peaks above intensity 150 taking 2.
The chromatographic separationwas accomplished ona kinetex c. The ab sciex tripletof 5600 system is designed for the qualitative and quantitative analysis of chemical species. Pacific biolabs purchases sciex tripletof 5600 high resolution. The results clearly demonstrate that accurate results can be. Industryleading toftof system technology from the company that the ab sciex toftof 5800 system continues the legacy of innovation that made ab sciex the world leader in life science mass spectrometry. Request pdf performance characteristics of a new hybrid quadrupole timeof flight tandem mass spectrometer tripletof 5600 the tripletof 5600. This section contains general safetyrelated information and provides regulatory compliance information. Quantification of five clinically important amino acids by. To test the quantitativeperformance of a absciex 5600 triple tof mass spectrometer workingin a pseudomrm mode and using pme8 qtapas sampleset as a test sample. Two samples from abrf sprg2011 were used for lc ms and ms ms by ab sciex 5600 tripletof with cid fragmentation. Chromatographic separation was conducted on a poroshell 120 ecc 18 2. Employing a saccharomyces cerevisiae tryptic digest, careful consideration of several performance. Glargine and its two metabolites m1 and m2 on triple quad 6500 and triple tof 5600 lc ms ms systems in a dog toxicokinetics study.
How to visualize ab sciex ttof 5600 data homesupport. In this study, a comparative uhplcq tof ms based metabolomics approach combining equivalentquantification and the taste activity value tav was used, for the first time, to evaluate the taste qualities and characterize metabolite profiles in chinese fish sauce during fermentation. Performance characteristics of a new hybrid quadrupole timeofflight tandem mass spectrometer tripletof 5600. The chromatographic separationwas accomplished ona kinetex c 18 100 mm 3. Msms, the mass window for precursor ion selection of the quadrupole mass analyzer was set.
A triple quadrupole mass spectrometer tqms, is a tandem mass spectrometer consisting of two quadrupole mass analyzers in series, with a nonmassresolving radio frequency rfonly quadrupole between them to act as a cell for collisioninduced dissociation. High throughput, data independent acquisition for qualitative. Timeofflight mass spectrometry was performed using an lctriple tof 5600. Fast and selective quantification of opiates in urine. On q exactive, the resolution was 70k for full scan and 35k for ms 2. The lc ms system consisted of an agilent 1100 series hplc agilent technologies, palo alto, ca,detectedusa coupled to a triple tof 5600 mass spectrometer sciex, ontario, canada equipped with a turboionspray source. Data acquisition and integration were controlled by analyst software. Our dedication to bringing you bestinindustry solutions is evident in our designs. It also describes potential hazards and associated warnings for the system and the precautions that should be taken to. Triple quad 5500 triple tof 5600 accurate mass lilly metabolomics platform.
Then the five most abundant peptides precursors that are not on exclusion list are selected for further fragmentation malditoftof analysis to generate the full scan mass spectrum. Click on the publication title for a direct link to the abstract. Agilent 6200 series tof and 6500 series q tof lc ms system concepts guide 11 a superheated sheath gas to collimate the nebulizer spray which dramatically increases the number of ions that enter the mass spectrometer. Studies on the metabolism and degradation of vancomycin in. Ion source gases gs1gs2 at 0, curtain gas cur at 10, temperature tem at 0 and nebulizer current nc at 3. M2 on triple quad 6500 and triple tof 5600 lcmsms systems in a dog toxicokinetics study. The tripletof 5600 system, a hybrid quadrupole timeofflight mass spectrometer, was evaluated to explore the key figures of merit in generating peptide and protein identifications that included. The tripletof 5600 system, a hybrid quadrupole timeofflight mass spectrometer, was evaluated to. No other accurate mass msms instrument has the speed and sensitivity to deliver comprehensive qualitative exploration, rapid profiling, and highresolution. Sample harvest and storage biological metadata sample extraction extraction metadata sample preparation. Help for applications you can use one or more of the agilent 6200 series tof or 6500. This application note presents the concept of multidimensional liquid chromatography ms mdlcmsbased lipidomics applied to lipid extracts from liver and skeletal muscle of mice fed a normal or a high fat diet, according to the multidimensional mass spectrometry mdmsbased shotgun lipidomics approach.
The system includes a mass spectrometer, a duospray ion source, the optional cds calibrant delivery system, and a computer running the analyst tf software. The tripletof 5600 system, a hybrid quadrupole timeofflight mass spectrometer, was evaluated to explore the key figures of merit in generating peptide and protein identifications that included spectral acquisition rates, data quality, proteome coverage, and biological depth. Infusion msmsall workflow experiments consisted of a tof ms scan from mz 2001500 followed by a sequential acquisition of 1001 msms spectra acquired from mz 200. On the navigation bar, under tune and calibrate, doubleclick manual tuning. We devised an analytical method for the detection of cardiac glycosides using lcqtofms and successfully detected the known cardenolidetype cardiac monoglycosides, cerberin, neriifolin, tanghinin. Before operating the system, carefully read all of the sections of this guide.
Performance characteristics of a new hybrid triple quadrupole. Multidimensional lcmsbased lipidomics using the sciex. High resolution quai and quant the ab sciex tripletof 5600 system is the first accuratemass, highresolution lcmsms system for qualitative analysis that has the speed and sensitivity to deliver quantitation like a highperformance triple quad. Jul 01, 2011 the recently introduced tripletof 5600 system tripletof, a hybrid triple quadrupole tof platform, is considered the first accurate mass, high resolution system of its kind and operates by means of information dependent acquisition ida with the speed and sensitivity of a tof ms and quantification capabilities similar to a triple quadrupole ms. This study shows the development, validation and application of a liquid chromatographyquadrupoletimeofflight mass spectrometry lcqtofms screening method for the. Uhplcqtofmsms analysis was performed on a shimadzu uhplc system shimadzu corp. Fast and selective quantification of opiates in urine using.